Journal: Human Reproduction (Oxford, England)

Article Title: Chronic hyperandrogenemia in the presence and absence of a western-style diet impairs ovarian and uterine structure/function in young adult rhesus monkeys

doi: 10.1093/humrep/dex338

Figure Lengend Snippet: Photomicrographs illustrating immunohistochemical staining for estrogen receptor 1 (ESR1), progesterone receptor (PGR) MMP26, TIMP3, Ki-67 and androgen receptor (AR) in the endometrial functionalis zone of the macaque uterus from representative females in each treatment group (C, T, WSD, T+WSD). Brown staining denotes positive expression of proteins. Sections are counterstained with hematoxylin (blue) staining. ESR1, PGR, Ki-67 and AR staining is nuclear, while MMP26 and TIMP3 show cytoplasmic localization. Inset shows a negative control with an irrelevant antibody (Anti-Br(d)U). TIMP3 staining was localized to the predecidual cells around the spiral arteries.

Article Snippet: Antibodies used were against estrogen receptor 1 (ESR1,ER-ID5; Cat#: MS-354-P, Thermo Fisher Scientific), progesterone receptor (PGR, Cat#: Ms-298-P, 1 μg, Lab Vision/NeoMarkers, Fremont, CA, USA), androgen receptor (AR-F39 (Cat#: MU256, 1/50, BioGenex, Fremont, CA, USA), Ki67 (Cat#: MU370-UC, 1/200, BioGenex), MMP26 (Cat# ab57636; Abcam, Cambridge, MA, USA) and TIMP3 (Cat#:MAB973, R&D Systems, Inc. Minneapolis, MN, USA).

Techniques: Immunohistochemical staining, Staining, Expressing, Negative Control

Journal: Human Reproduction (Oxford, England)

Article Title: Chronic hyperandrogenemia in the presence and absence of a western-style diet impairs ovarian and uterine structure/function in young adult rhesus monkeys

doi: 10.1093/humrep/dex338

Figure Lengend Snippet: Expression of ESR1, PGR, MMP26 and TIMP3 mRNAs as detected by qRT-PCR in endometrial biopsies collected from macaques in the mid-luteal phase of the cycle. Significant (P < 0.05) differences between individual treatment groups are denoted by different uppercase letters above columns.

Article Snippet: Antibodies used were against estrogen receptor 1 (ESR1,ER-ID5; Cat#: MS-354-P, Thermo Fisher Scientific), progesterone receptor (PGR, Cat#: Ms-298-P, 1 μg, Lab Vision/NeoMarkers, Fremont, CA, USA), androgen receptor (AR-F39 (Cat#: MU256, 1/50, BioGenex, Fremont, CA, USA), Ki67 (Cat#: MU370-UC, 1/200, BioGenex), MMP26 (Cat# ab57636; Abcam, Cambridge, MA, USA) and TIMP3 (Cat#:MAB973, R&D Systems, Inc. Minneapolis, MN, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: BMC Cancer

Article Title: Remodeling of extracellular matrix by normal and tumor-associated fibroblasts promotes cervical cancer progression

doi: 10.1186/s12885-015-1272-3

Figure Lengend Snippet: Antibodies used in the present study

Article Snippet: TIMP3 (D74B10) , Rabbit monoclonal IgG , Cell Signaling , 5673 , - , 1:1000.

Techniques:

Journal: Scientific reports

Article Title: WNTA5-mediated miR-374a-5p regulates vascular smooth muscle cell phenotype transformation and M1 macrophage polarization impacting intracranial aneurysm progression.

doi: 10.1038/s41598-024-51243-z

Figure Lengend Snippet: Figure 1. miR-374a-5p regulated VSMC phenotypic transformation (A) qRT-PCR analysis of miR-374a-5p expression. (B) IF double-staining detection of contractile markers (SM22α and α-SMA) expression in VSMCs. Scale bar = 25 μm. (C) WB analysis of SM22α, α-SMA, Calponin, MMP1, MMP3, MMP9, TIMP3, and TIMP4 expression. (D) ELISA measurement of TNF-α, IL-1β, IL-6, and IL-10 levels. *P < 0.05 vs. Control, &P < 0.05 vs. inhibitor NC. (E) qRT-PCR analysis of miR-374a-5p expression. (F) IF double-staining detection of SM22α and α-SMA expression in VSMCs. Scale bar = 25 μm. *P < 0.05 vs. Control, &P < 0.05 vs. mimics NC. n = 3.

Article Snippet: Indicator Dilution Origin Manufacturer Calponin 1:5000 Rabbit 24855-1-AP, Proteintech TIMP3 1:1000 Rabbit 10858-1-AP, Proteintech TIMP4 1:1000 Rabbit 12326-1-AP, Proteintech SM22α 1:5000 Rabbit ab155272, Abcam α-SMA 1 μg/mL Rabbit ab5694, Abcam MMP1 1:1000 Rabbit 26585-1-AP, Proteintech MMP3 1:1000 Rabbit ab53015, Abcam MMP9 1:1000 Rabbit 27306-1-AP, Proteintech WNT2B 1:3000 Rabbit ab178418, Abcam WNT3 1 μg/mL Rabbit ab32249, Abcam WNT5A 1:3000 Rabbit ab235966, Abcam GAPDH 1:5000 Rabbit 10494-1-AP, Proteintech HRP goat anti-mouse IgG 1:5000 Mouse SA00001-1, Proteintech HRP goat anti-rabbit IgG 1:6000 Rabbit SA00001-2, Proteintech 5 Vol.

Techniques: Transformation Assay, Quantitative RT-PCR, Expressing, Double Staining, Enzyme-linked Immunosorbent Assay, Control

Journal: Scientific reports

Article Title: WNTA5-mediated miR-374a-5p regulates vascular smooth muscle cell phenotype transformation and M1 macrophage polarization impacting intracranial aneurysm progression.

doi: 10.1038/s41598-024-51243-z

Figure Lengend Snippet: Figure 2. In vivo validation of miR-374a-5p affecting vascular remodeling in IA rats (A) and (B) SBP and DBP in each treatment group of mice at weeks 0, 1, 2, 3, and 4 were recorded. (C) HE staining to observe vascular pathological morphology. Scale bar = 100 μm and 2000 μm. (D) and (E) qRT-PCR analysis of miR-374a-5p expression in vascular wall tissue and plasma of IA rats. (F) The co-localization and expression of miR-374a-5b and SM22α in arterial wall were detected by FISH-IF. Scale bar = 25 μm. (G) WB analysis of SM22α, α-SMA, Calponin, MMP1, MMP3, MMP9, TIMP3, and TIMP4 expression. (H) Flow cytometry to detect infiltration of M1 macrophages (CD86) and M2 macrophages (CD206). (I) ELISA measurement of TNF-α, IL-1β, IL-6, and IL-10 levels. *P < 0.05 vs. Sham, &P < 0.05 vs. IA + inhibitor NC. n = 3.

Article Snippet: Indicator Dilution Origin Manufacturer Calponin 1:5000 Rabbit 24855-1-AP, Proteintech TIMP3 1:1000 Rabbit 10858-1-AP, Proteintech TIMP4 1:1000 Rabbit 12326-1-AP, Proteintech SM22α 1:5000 Rabbit ab155272, Abcam α-SMA 1 μg/mL Rabbit ab5694, Abcam MMP1 1:1000 Rabbit 26585-1-AP, Proteintech MMP3 1:1000 Rabbit ab53015, Abcam MMP9 1:1000 Rabbit 27306-1-AP, Proteintech WNT2B 1:3000 Rabbit ab178418, Abcam WNT3 1 μg/mL Rabbit ab32249, Abcam WNT5A 1:3000 Rabbit ab235966, Abcam GAPDH 1:5000 Rabbit 10494-1-AP, Proteintech HRP goat anti-mouse IgG 1:5000 Mouse SA00001-1, Proteintech HRP goat anti-rabbit IgG 1:6000 Rabbit SA00001-2, Proteintech 5 Vol.

Techniques: In Vivo, Biomarker Discovery, Staining, Quantitative RT-PCR, Expressing, Clinical Proteomics, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Scientific reports

Article Title: WNTA5-mediated miR-374a-5p regulates vascular smooth muscle cell phenotype transformation and M1 macrophage polarization impacting intracranial aneurysm progression.

doi: 10.1038/s41598-024-51243-z

Figure Lengend Snippet: Figure 4. WNT5A-mediated miR-374a-5p affected VSMC phenotypic transformation (A) WB detection of intracellular WNT5A signal expression. (B) IF double-staining to detect the expression of smooth muscle cell contraction markers (SM22α and α-SMA). Scale bar = 25 μm. (C) WB detection of SM22α, α-SMA, Calponin, MMP1, MMP3, MMP9, TIMP3, and TIMP4 expression. (D) ELISA measurement of TNF-α, IL-1β, IL-6, and IL-10 levels. *P < 0.05 vs. inhibitor NC. &P < 0.05 vs. miR-374a-5p inhibitor + Vehicle. n = 3.

Article Snippet: Indicator Dilution Origin Manufacturer Calponin 1:5000 Rabbit 24855-1-AP, Proteintech TIMP3 1:1000 Rabbit 10858-1-AP, Proteintech TIMP4 1:1000 Rabbit 12326-1-AP, Proteintech SM22α 1:5000 Rabbit ab155272, Abcam α-SMA 1 μg/mL Rabbit ab5694, Abcam MMP1 1:1000 Rabbit 26585-1-AP, Proteintech MMP3 1:1000 Rabbit ab53015, Abcam MMP9 1:1000 Rabbit 27306-1-AP, Proteintech WNT2B 1:3000 Rabbit ab178418, Abcam WNT3 1 μg/mL Rabbit ab32249, Abcam WNT5A 1:3000 Rabbit ab235966, Abcam GAPDH 1:5000 Rabbit 10494-1-AP, Proteintech HRP goat anti-mouse IgG 1:5000 Mouse SA00001-1, Proteintech HRP goat anti-rabbit IgG 1:6000 Rabbit SA00001-2, Proteintech 5 Vol.

Techniques: Transformation Assay, Expressing, Double Staining, Enzyme-linked Immunosorbent Assay